Cambridge AssessmentCANDIDATENAMECENTRECANDIDATENUMBERNUMBERBIOLOGY0610/62February/March 20231 hourYou must answer on the question paper.No additional materials are needed.INSTRUCTIONS.Answer all questions.Use a black or dark blue pen.You may use an HB pencil for any diagrams or graphs.Write your name,centre number and candidate number in the boxes at the top of the page.Write your answer to each question in the space provided.Do not use an erasable pen or correction fluid.Do not write on any bar codes.You may use a calculator.You should show all your working and use appropriate units.INFORMATION.The total mark for this paper is 40.The number of marks for each question or part question is shown in brackets ]This document has 16 pages.Any blank pages are indicated.DC(PQ/JG)312355/3©UCLES2023[Turn over1 Potato cells take up methylene blue dye when the potato tissue is placed into the dye solution.If the dyed potato cells are placed in water the dye will diffuse into the water.A student investigated the effect of temperature on the diffusion of methylene blue dye from dyedpotato cells.The student used this method:Step 1 You are provided with a beaker of cold water,a beaker of warm water and a beaker ofhot water.Step 2 Measure the temperature of the water in the beaker of hot water.Step 3 Label one test-tube C,one test-tube W and one test-tube H.Step 4 Draw a line 9cm from the base of each test-tube,as shown in Fig.1.1.Place the threetest-tubes in a test-tube rack.line drawn9cmFig.1.1Step 5 Fill test-tube C with cold water up to the line drawn in step 4.Put test-tube C into thebeaker of cold water.Step 6 Fill test-tube W with warm water up to the line drawn in step 4.Put test-tube W into thebeaker of warm water.Step 7 Fill test-tube H with hot water up to the line drawn in step 4.Put test-tube H into thebeaker of hot water.Step 8 You are provided with three potato cylinders.They have been soaked in methylene bluesolution and then rinsed.Place the three potato cylinders on the white tile and cut the three potato cylinders toapproximately 2cm in length.Step 9 Place one of the potato cylinders from step 8 into each test-tube.Step 10 Start the stop-clock.Leave the test-tubes for 15 minutes.Step 11 After 15 minutes,measure the temperature of the water in the beaker of hot water again.©UCLES20230610/62/F/M233Fig.1.2 shows part of the thermometer during step 2 and step 11.7065356030step 2step 11Fig.1.2(a)(i)Record the temperatures shown on the thermometer for step 2 and step 11 in Fig.1.2.water temperature in the beaker of hot water in step 2 ...............................................cwater temperature in the beaker of hot water in step 11.............................cStep 12 Remove the test-tubes C,W and H from the beakers and shake all the test-tubes for10 seconds.Place the test-tubes into the test-tube rack.Step 13 Observe the intensity of the blue colour of the liquid in test-tubes C,W and H.©UCLES20230610/62/F/M23